Testing Plant Responses to Rarified Atmospheres for Inflatable Greenhouses

Reduced atmospheric pressures will likely be used to minimize mass and engineering requirements for plant growth habitats used in extraterrestrial applications. A chamber with high vacuum capability was used to design and begin construction of a system for testing plant responses to reduced pressure atmospheres. Several preliminary tests were conducted to evaluate chamber suitability for plant tests and to determine performance of thermal and vacuum systems at ambient and reduced pressure atmospheres down to 0.1 atm. The first tests consisted of measurements of internal gas volume and leakage rate. The method for volume determination was quite sensitive and will be needed for plant gas exchange measurements and calculations. This information will also be used in conjunction with the leak rate. Measured leak rates on the order of 0.46 mm Hg/min at 76 mm Hg pressure are low enough to conduct sensitive carbon dioxide exchange rate measurements at reduced pressure given an adequate plant sample (0.5 to 1.0 sq m area). A test rack with lighting provided by three high-pressure sodium vapor lamps was built to accommodate both short-term and long-term plant responses. Initial short-term experiments with lettuce showed that a pressure of 77 mm Hg resulted in a 6.1-fold increase in the rate of water loss compared to water loss at ambient pressure. Plants were severely wilted after 30 minutes exposure to 77 mm Hg. Water loss was found to be inversely correlated with atmospheric pressure over the range of pressures from 0.2 to 1.0 atm; the rate of water loss at 0.2 atm was 4.3 times higher than water loss at ambient pressure. Older leaves showed moderate wilting during exposure to 156 mm Hg, but those exposed to 345 mm, Hg remained turgid. Results suggest a reduced atmospheric pressure limit of 0.2 to 0.3 atm for lettuce grown in a solid medium. Follow-up experiments with carbon dioxide control and control at high relative humidity (> 90 %) will be needed to further confirm and define safe reduced pressure limits that are feasible for plant tolerance and growth.