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Fluorescence activated cell sorting.An instrument has been developed for sorting biological cells. The cells are rendered differentially fluorescent and incorporated into a small liquid stream illuminated by a laser beam. The cells pass sequentially through the beam, and fluorescent light from the cells gives rise to electrical signals. The stream is broken into a series of uniform size drops downstream of the laser. The cell signals are used to give appropriate electrostatic charges to drops containing the cells. The drops then pass between two charged plates and are deflected to appropriate containers. The system has proved capable of providing fractions containing large numbers of viable cells highly enriched in a particular functional type.
Document ID
19720039737
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Bonner, W. A.
Hulett, H. R.
Sweet, R. G.
Herzenberg, L. A.
(Stanford University Stanford, Calif., United States)
Date Acquired
August 6, 2013
Publication Date
March 1, 1972
Publication Information
Publication: Review of Scientific Instruments
Volume: 43
Subject Category
Biotechnology
Accession Number
72A23403
Funding Number(s)
CONTRACT_GRANT: NIH-GM-17367
CONTRACT_GRANT: NGR-05-020-004
Distribution Limits
Public
Copyright
Other

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