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Quantitative bioluminescent detection of bacteriaPhosphoflavins in sample are measured using photobacterial luciferase assay technique for flavin mononucleotide (FMN). Boiling perchloric acid is used to rupture cells to free bound flavin and to hydrolyze flavin adenine dinucleotide to FMN. Base-stabilized water solution of sodium borohydride is used as reactant.
Document ID
19760000073
Acquisition Source
Legacy CDMS
Document Type
Other - NASA Tech Brief
Authors
Chappelle, E. W.
Picciolo, G. L.
Date Acquired
August 8, 2013
Publication Date
March 1, 1976
Publication Information
Publication: NASA Tech Briefs
Volume: 1
Issue: 1
ISSN: 0145-319X
Subject Category
Life Sciences
Report/Patent Number
GSC-12003
Accession Number
76B10073
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.

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