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Factors affecting the palmitoyl-coenzyme A desaturase of Saccharomyces cerevisiaeThe activity and stability of the palmitoyl-coenzyme A (CoA) desaturase complex of Saccharomyces cerevisiae was influenced by several factors. Cells, grown nonaerobically and then incubated with glucose, either in air or under N2, showed a marked increase in desaturase activity. Cycloheximide, added during such incubations, prevented the increase in activity, suggesting de novo synthesis. The stability of the desaturase from cells grown nonaerobically was affected by subsequent treatment of the cells; enzyme from freshly harvested cells, or from cells that were then shaken under nitrogen, readily lost activity upon washing or during density gradient analysis, whereas aerated cells, in the presence or absence of glucose, yielded stable enzyme preparations. The loss of activity in nonaerobic preparations could be reversed by adding soluble supernatant from these homogenates and could be prevented by growing the cells in the presence of palmitoleic acid and ergosterol, but not with several other lipids tested.
Document ID
19760038826
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Klein, H. P.
(NASA Ames Research Center Moffett Field, CA, United States)
Volkmann, C. M.
(NASA Ames Research Center Planetary Biology Div., Moffett Field, Calif., United States)
Date Acquired
August 8, 2013
Publication Date
November 1, 1975
Publication Information
Publication: Journal of Bacteriology
Volume: 124
Subject Category
Life Sciences (General)
Accession Number
76A21792
Distribution Limits
Public
Copyright
Other

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