NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
Streaming potential measurements of biosurfacesA technique based on the measurement of streaming potentials has been developed to evaluate the electrokinetic region of the cell periphery. This approach is feasible for cell lines propagated in in-vitro cell cultures in monolayer form. The advantage of this system is that cells may be evaluated in the living state atttached to a substrate; it is not necessary to subject the cells to enzymatic, chemical, or mechanical trauma required to obtain monodisperse suspensions which are then normally evaluated by microelectrophoresis. In this manner, it should be possible to study the influence of substrate and environmental factors on the charge density and potential at the cell periphery. The apparatus and procedure are described as well as some results concerning the electrokinetic potential of borosilicate capillaries as a function of ionic strength, pH, and temperature. The effect that turbulence and entrance flow conditions have on accurate streaming-potential measurements is discussed. The electrokinetic potential of BALB/c 3T12 fibroblasts has been quantified as a function of pH, ionic strength, glutaraldehyde fixation, and Giemsa staining.
Document ID
19760064124
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Van Wagenen, R. A.
(Utah Univ. Salt Lake City, UT, United States)
Andrade, J. D.
(Utah, University Salt Lake City, Utah, United States)
Hibbs, J. B., Jr.
(U.S. Veterans Administration Hospital; Utah, University, Medical Center Salt Lake City, Utah, United States)
Date Acquired
August 8, 2013
Publication Date
October 1, 1976
Subject Category
Chemistry And Materials (General)
Accession Number
76A47090
Funding Number(s)
CONTRACT_GRANT: NIH-CA-15811
CONTRACT_GRANT: NSF GH-38996X
CONTRACT_GRANT: NAS8-30253
Distribution Limits
Public
Copyright
Other

Available Downloads

There are no available downloads for this record.
No Preview Available