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Microbial metabolic activity in soil as measured by dehydrogenase determinationsThe dehydrogenase technique for measuring the metabolic activity of microorganisms in soil was modified to use a 6-h, 37 C incubation with either glucose or yeast extract as the electron-donating substrate. The rate of formazan production remained constant during this time interval, and cellular multiplication apparently did not occur. The technique was used to follow changes in the overall metabolic activities of microorganisms in soil undergoing incubation with a limiting concentration of added nutrient. The sequence of events was similar to that obtained by using the Warburg respirometer to measure O2 consumption. However, the major peaks of activity occurred earlier with the respirometer. This possibly is due to the lack of atmospheric CO2 during the O2 consumption measurements.
Document ID
19780043989
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Casida, L. E., Jr.
(Pennsylvania State University University Park, Pa., United States)
Date Acquired
August 9, 2013
Publication Date
December 1, 1977
Publication Information
Publication: Applied and Environmental Microbiology
Volume: 34
Subject Category
Chemistry And Materials (General)
Accession Number
78A27898
Funding Number(s)
CONTRACT_GRANT: NGR-39-009-180
Distribution Limits
Public
Copyright
Other

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