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Preparative cell electrophoresis at 1 and 0 gravityIt is attempted to show that the use of heavy water (D2O) as starting cushion for the cells combines the advantages of the required density difference, with no lasting biochemical or physiochemical influence on the cells. Phosphate buffers of low ionic strength were prepared in distilled water or heavy water. A vertical starch gel electrophoresis was used to support a cylindrical polystyrene electrophoresis tube used for lymphocyte separations, 25 cm in length and 0.75 cm I.D., prepared from a 10 ml disposable pipet. Erythrocyte separations were carried out in a jacketed rectangular plexiglas chamber. It is pointed out that the described preparative D2O gradient electrophoresis method cannot be readily used for the measurement of electrophoretic mobilities for analytical purposes. However, for the preparative separation of cells with only slightly different electrokinetic properties the method appears promising, simple, and entirely inocuous to the cells.
Document ID
19790035650
Acquisition Source
Legacy CDMS
Document Type
Conference Paper
Authors
Van Oss, C. J.
(State Univ. of New York Buffalo, NY, United States)
Bronson, P. M.
(New York, State University Buffalo, N.Y., United States)
Date Acquired
August 9, 2013
Publication Date
January 1, 1979
Subject Category
Astronautics (General)
Report/Patent Number
AIAA PAPER 79-0309
Meeting Information
Meeting: American Institute of Aeronautics and Astronautics, Aerospace Sciences Meeting
Location: New Orleans, LA
Start Date: January 15, 1979
End Date: January 17, 1979
Sponsors: American Institute of Aeronautics, Astronautics
Accession Number
79A19663
Funding Number(s)
CONTRACT_GRANT: NAS8-32947
Distribution Limits
Public
Copyright
Other

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