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Polyglutaraldehyde - A new reagent for coupling proteins to microspheres and for labeling cell-surface receptorsGlutaraldehyde polymerized in basic aqueous solutions was found to react with low molecular weight amines, immunoglobulins and hemoglobin. The polyglutaraldehyde was covalently bound to hydrophilic microspheres. The rate of addition of proteins to the polyglutaraldehyde-derivatized microspheres was investigated spectrophotometrically as a function of pH and temperature. The reaction of polyglutaraldehyde was found to be faster than that of the monomer. The findings led to successful labeling of human lymphocyte subpopulations.
Document ID
19790066217
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Rembaum, A.
(California Institute of Technology, Jet Propulsion Laboratory, Pasadena Calif., United States)
Levy, J.
(California Institute of Technology, Jet Propulsion Laboratory, Pasadena; California, University Los Angeles, Calif., United States)
Margel, S.
(Jet Propulsion Lab., California Inst. of Tech. Pasadena, CA, United States)
Date Acquired
August 9, 2013
Publication Date
January 1, 1978
Publication Information
Publication: Journal of Immunological Methods
Volume: 24
Subject Category
Life Sciences (General)
Accession Number
79A50230
Funding Number(s)
CONTRACT_GRANT: NIH-1-R01-CA-20668-01
Distribution Limits
Public
Copyright
Other

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