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Microdisc gel electrophoresis in sodium dodecyl sulfate of organic material from rat otoconial complexesThe gravity receptors of all vertebrates utilize a 'test mass' consisting of a complex arrangement of mineral and organic substance that lies over the sensory receptor areas. In most vertebrates, the mineral is a polymorph of calcium carbonate in the form of minute, single crystals called otoconia. An investigation is conducted to determine the number of proteins in otoconial complexes and their molecular weights. The investigation makes use of a microdisk gel electrophoresis method reported by Gainer (1971). The most important finding of the reported research is that analysis of the proteins of the organic material of the otoconial complexes is possible when sensitive microanalytical methods are employed. Further modification of the basic technique employed and the inclusion of other sensitive staining methods should mean that, in the future, protein separation by molecular weight will be possible in sample pools containing only two otoconial masses.
Document ID
19820033947
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Ross, M. D.
(Michigan Univ. Ann Arbor, MI, United States)
Pote, K. G.
(Michigan Univ. Ann Arbor, MI, United States)
Rarey, K. E.
(Michigan Univ. Ann Arbor, MI, United States)
Verma, L. M.
(Michigan, University Ann Arbor, MI, United States)
Date Acquired
August 10, 2013
Publication Date
November 6, 1981
Subject Category
Life Sciences (General)
Accession Number
82A17482
Funding Number(s)
CONTRACT_GRANT: NIH-AG-00767
CONTRACT_GRANT: NSG-9047
Distribution Limits
Public
Copyright
Other

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