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The effects of cutting or of stretching skeletal muscle in vitro on the rates of protein synthesis and degradationSkeletal muscle preparations using cut muscle fibers have often been used in studies of protein metabolism. The present paper reports an investigation of the effect of muscle cutting or stretching in vitro on the rates of protein synthesis and/or degradation. Protein synthesis and content, and ATP and phosphocreatine levels were monitored in soleus and extensor digitorum longus muscles from the rat with various extents of muscle fiber cuts and following stretching to about 120% the resting length. Rates of protein synthesis are found to be significantly lower and protein degradation higher in the cut muscles than in uncut controls, while ATP and phosphocreatine concentrations decreased. Stretched intact muscles, on the other hand, are observed to have higher concentrations of high-energy phosphates than unstretched muscles, while rates of protein degradation were not affected. Results thus demonstrate that the cutting of skeletal muscle fibers alters many aspects of muscle metabolism, and that moderate decreases in ATP concentration do not alter rates of protein concentration in intact muscles in vitro.
Document ID
19820039709
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Seider, M. J.
(Texas Univ. Houston, TX, United States)
Kapp, R.
(Texas Univ. Houston, TX, United States)
Chen, C.-P.
(Texas Univ. Houston, TX, United States)
Booth, F. W.
(Texas, University Houston, TX, United States)
Date Acquired
August 10, 2013
Publication Date
January 1, 1980
Publication Information
Publication: Biochemical Journal
Volume: 188
Subject Category
Life Sciences (General)
Accession Number
82A23244
Funding Number(s)
CONTRACT_GRANT: NAS9-15388
CONTRACT_GRANT: PHS-AM-19393
Distribution Limits
Public
Copyright
Other

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