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Studies with sample conductivity, insertion rates, and particle deflection in a continuous flow electrophoresis systemThe continuous flow electrophoresis system makes electrophoresis possible in a free-flowing film of aqueous electrolyte medium. The sample continuously enters the electrolyte at the top of the chamber and is subjected to the action of a lateral dc field. This divides the sample into fractions since each component has a distinctive electrophoretic mobility. Tests were made using monodisperse polystyrene latex microspheres to determine optimum sample conductivity, insertion rates and optimum electric field applications as baseline data for future STS flight experiments. Optimum sample flow rates for the selected samples were determined to be approximately 26 micro-liters/min. Experiments with samples in deionized water yielded best results and voltages in the 20 V/cm to 30 V/cm range were optimum. Deflections of formaldehyde fixed turkey and bovine erythrocytes were determined using the continuous flow electrophoresis system. The effects of particle interactions on sample resolution and migration in the chamber was also evaluated.
Document ID
19830009131
Acquisition Source
Legacy CDMS
Document Type
Conference Paper
Authors
Williams, G., Jr.
(Calhoun Community Coll. Decatur, AL, United States)
Date Acquired
August 11, 2013
Publication Date
August 1, 1982
Publication Information
Publication: NASA. Marshall Space Flight Center The 1982 NASA(ASEE Summer Fac. Fellowship Program
Subject Category
Inorganic And Physical Chemistry
Accession Number
83N17402
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.

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