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Removal of histological sections from glass for electron microscopy - Use of Quetol 651 resin and heatA number of approaches have been used to separate stained conventional histological sections from glass slides in preparation for a study with the electron microscope. However, in each reported case some problems were encountered with respect to the separation process. The present investigation is concerned with the use of the epoxy resin Quetol 651 as an embedding medium for this procedure, taking into account the simple application of heat (62-64 C) for performing the separation step. After the tissue has been removed from the glass by the considered technique, it is thin sectioned, and stained with uranyl acetate-lead citrate.
Document ID
19830065491
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Kraft, L. M.
(NASA Ames Research Center Biomedical Research Div., Moffett Field, CA, United States)
Joyce, K.
(NASA Ames Research Center Moffett Field, CA, United States)
Dantoni Damelio, E.
(San Francisco, University San Francisco, CA, United States)
Date Acquired
August 11, 2013
Publication Date
January 1, 1983
Publication Information
Publication: Stain Technology
Volume: 58
Issue: 1, 19
ISSN: 0038-9153
Subject Category
Life Sciences (General)
Accession Number
83A46709
Distribution Limits
Public
Copyright
Other

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