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Pea amyloplast DNA is qualitatively similar to pea chloroplast DNAAmyloplast DNA (apDNA), when subjected to digestion with restriction endonucleases, yields patterns nearly identical to that of DNA from mature pea chloroplasts (ctDNA). Southern transfers of apDNA and ctDNA, probed with the large subunit (LS) gene of ribulose-1,5-bisphosphate carboxylase (Rubisco), shows hybridization to the expected restriction fragments for both apDNA and ctDNA. However, Northern transfers of total RNA from chloroplasts and amyloplasts, probed again with the LS gene of Rubisco, shows that no detectable LS meggage is found in amyloplasts although LS expression in mature chloroplasts is high. Likewise, two dimensional polyacrylamide gel electrophoresis of etiolated gravisensitive pea tissue shows that both large and small subunits of Rubisco are conspicuously absent; however, in greening tissue these two constitute the major soluble proteins. These findings suggest that although the informational content of these two organelle types is equivalent, gene expression is quite different and is presumably under nuclear control.
Document ID
19840010809
Acquisition Source
Legacy CDMS
Document Type
Conference Paper
Authors
Gaynor, J. J.
(Rockefeller Univ. New York, NY, United States)
Date Acquired
August 12, 2013
Publication Date
February 1, 1984
Publication Information
Publication: NASA. Washington NASA Space Biol. Program
Subject Category
Life Sciences (General)
Accession Number
84N18877
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.

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