Clinostat effects on shoot and root of Arabidopsis

In the clinostat environment, Arabidopsis thaliana (L.) Heynh. plants grown in cultures having limited gas exchange with the external atmosphere developed 'above' the agar media a large mass of roots which eventually covered the stem. Cultural conditions were 0.5 rpm, 350 ft.c. from cool white fluorescent lamps, and 25 C. Limited gas exchange culture tubes were covered with Saran Wrap or mylar film, while free gas exchange cultures were plugged with cotton or polyurethane foam. Average shoot-root dry weight ratio of clinostatted, limited gas exchange plants was 3.39 (p less than 0.001) as compared to 10.1 for upright stationary and 10.3 for vertically rotated controls. Average dry weight (48.3 mg) of all clinostatted shoots was greater than those reported by other investigators (15.1 mg). Finally, shoots of the clinostatted plants reported here were almost 2x heavier (p less than 0.01) than the controls (28.7 mg, 26.7 mg), a result contrary to findings of previous investigators, where the clinostatted plants are the lightest (clinostat 15.1 mg, controls 16.5 mg and 17.2 mg).