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Intracellular coagulation inhibits the extraction of proteins from ProchloronProtein extraction from the prokaryotic alga Prochloron LP (isolated from the ascidian host Lissoclinum patella) was complicated by an irreversible loss of cell fragility in the isolated algae. Accompanying this phenomenon, which is termed intracellular coagulation, was a redistribution of thylakoids around the cell periphery, a loss of photosynthetic O2 production, and a drastic decrease in the extractability of cell proteins. Procedures are described for the successful preparation and transport of cell extracts yielding the enzymes glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase as well as other soluble proteins.
Document ID
19840046827
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Fall, R.
(Colorado, University; Cooperative Institute for Research in Environmental Sciences, Boulder, CO, United States)
Lewin, R. A.
(California, University La Jolla, CA, United States)
Fall, L. R.
(Colorado, University Boulder, CO, United States)
Date Acquired
August 12, 2013
Publication Date
January 1, 1983
Publication Information
Publication: Phytochemistry
Volume: 22
Issue: 11 1
ISSN: 0031-9422
Subject Category
Life Sciences (General)
Accession Number
84A29614
Funding Number(s)
CONTRACT_GRANT: NAGW-181
Distribution Limits
Public
Copyright
Other

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