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Rapid diagnostic methods for influenza virus in clinical specimens - A comparative studyA comparison of five rapid viral diagnostic techniques for identifying influenza virus in nasopharyngeal aspirates has been made on patients with influenza-like illnesses. Initial results with immune electron microscopy were positive in only one of 11 specimens from which virus was isolated and further work abandoned. Four other rapid tests were carried out on 39 specimens from which influenza virus had been isolated in tissue culture in 28. Of these 28 specimens yielding virus, 24 (85.7 percent) were positive by an indirect fluorescent antibody test (IFAT) on nasopharyngeal cells, 18 (64.3 percent) by enzyme-linked immunosorbent assay (ELISA), 19 (67.8 percent) by enzyme-linked fluorescent assay (ELFA), and 26 (92.8 percent) by a rapid tissue culture amplification method (TCA) in a continuous Rhesus monkey kidney line (LLC-MK2) with identification of virus by fluorescent antibody. In terms of sensitivity, simplicity, and rapidity, a combination of the IFAT and TCA methods seems to be very useful.
Document ID
19840062761
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Evans, A. S.
(Yale Univ. New Haven, CT, United States)
Olson, B.
(Yale University New Haven, CT, United States)
Date Acquired
August 12, 2013
Publication Date
January 1, 1982
Publication Information
Publication: Yale Journal of Biology and Medicine
Volume: 55
ISSN: 0044-0086
Subject Category
Aerospace Medicine
Accession Number
84A45548
Funding Number(s)
CONTRACT_GRANT: NSG-2185
Distribution Limits
Public
Copyright
Other

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