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Electrophoretic mobilities of erythrocytes in various buffersThe calibration of space flight equipment depends on a source of standard test particles, this test particle of choice is the fixed erythrocyte. Erythrocytes from different species have different electrophoretic mobilities. Electrophoretic mobility depends upon zeta potential, which, in turn depends upon ionic strength. Zeta potential decreases with increasing ionic strength, so cells have high electrophoretic mobility in space electrophoresis buffers than in typical physiological buffers. The electrophoretic mobilities of fixed human, rat, and rabbit erythrocytes in 0.145 M salt and buffers of varying ionic strength, temperature, and composition, to assess the effects of some of the unique combinations used in space buffers were characterized. Several effects were assessed: glycerol or DMSO (dimethylsulfoxide) were considered for use as cryoprotectants. The effect of these substances on erythrocyte electrophoretic mobility was examined. The choice of buffer depended upon cell mobility. Primary experiments with kidney cells established the choice of buffer and cryoprotectant. A nonstandard temperature of EPM in the suitable buffer was determined. A loss of ionic strength control occurs in the course of preparing columns for flight, the effects of small increases in ionic strength over the expected low values need to be evaluated.
Document ID
19850023443
Acquisition Source
Legacy CDMS
Document Type
Other
Authors
Plank, L. D.
(Pennsylvania State Univ. University Park, PA, United States)
Kunze, M. E.
(Pennsylvania State Univ. University Park, PA, United States)
Todd, P. W.
(Pennsylvania State Univ. University Park, PA, United States)
Date Acquired
August 12, 2013
Publication Date
January 1, 1985
Publication Information
Publication: Kidney Cell Electrophoresis
Subject Category
Inorganic And Physical Chemistry
Accession Number
85N31756
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.

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