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Effects of method of detachment on electrophoretic mobility of mammalian cells grown in monolayer cultureA variety of proteolytic and micolytic enzumes, mechanical procedures, and changes in the ionic environment, especially Ca chelation, are used for dispersal of monolayer grown cells. If either chelating agents or mechanical dispersion are used alone, the cell yield is often low and suspensions of single cells are difficult to obtain. Confluent monolayers treated with EDTA tend to be released from their surfaces in sheets, and clumps of cells remain even after further incubation in EDTA. Crude trypsin is the most popular dispersal agent and is known to contain a variety of contaminating enzymes which contribute to the dispersal of cells. A variety of cell injuries resulting from the activity of proteolytic enzymes are reported. It is shown that crystalline trypsin is least harmful to cell integrity as judged by trypan blue uptake.
Document ID
19850023444
Acquisition Source
Legacy CDMS
Document Type
Other
Authors
Plank, L. D.
(Pennsylvania State Univ. University Park, PA, United States)
Kunze, M. E.
(Pennsylvania State Univ. University Park, PA, United States)
Todd, P. W.
(Pennsylvania State Univ. University Park, PA, United States)
Date Acquired
August 12, 2013
Publication Date
January 1, 1985
Publication Information
Publication: Kidney Cell Electrophoresis
Subject Category
Inorganic And Physical Chemistry
Accession Number
85N31757
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.

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