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Size and DNA distributions of electrophoretically separated cultured human kidney cellsElectrophoretic purification of purifying cultured cells according to function presumes that the size of cycle phase of a cell is not an overriding determinant of its electrophoretic velocity in an electrophoretic separator. The size distributions and DNA distributions of fractions of cells purified by density gradient electrophoresis were determined. No systematic dependence of electrophoretic migration upward in a density gradient column upon either size or DNA content were found. It was found that human leukemia cell populations, which are more uniform function and found in all phases of the cell cycle during exponential growth, separated on a vertical sensity gradient electrophoresis column according to their size, which is shown to be strictly cell cycle dependent.
Document ID
19850023446
Acquisition Source
Legacy CDMS
Document Type
Other
Authors
Kunze, M. E.
(Pennsylvania State Univ. University Park, PA, United States)
Plank, L. D.
(Pennsylvania State Univ. University Park, PA, United States)
Todd, P. W.
(Pennsylvania State Univ. University Park, PA, United States)
Date Acquired
August 12, 2013
Publication Date
January 1, 1985
Publication Information
Publication: Kidney Cell Electrophoresis
Subject Category
Inorganic And Physical Chemistry
Accession Number
85N31759
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.

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