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pH profile of the adsorption of nucleotides onto montmorillonite. II - Adsorption and desorption of 5-prime-AMP in iron-calcium montmorillonite systemsThe interaction of 5-prime-AMP with montmorillonite saturated with various ratios of two metals found ubiquitously on the surface of earth, that is, iron and calcium, is investigated. Adsorption and desorption of the nucleotide were studied in the pH range of 2-12 at three levels of addition: 0.080, 0.268 and 0.803 mmole 5-prime-AMP per gram of clay. Two desorption stages were employed - H2O wash and NaOH extraction (pH = 12.0). 5-prime-AMP was preferentially adsorbed on the Fe-containing clays relative to the Ca clay. The nucleotide was fully recovered by the two desorption stages, mostly by the NaOH extraction. The evidence at hand indicates that 5-prime-AMP reaction with clay is affected by electrostatic interactions involving both attraction and repulsion forces. Some specific adsorption, possibly the result of covalent bonding and complex formation with the adsorbed ion, cannot be ruled out for iron but does not appear to operate for calcium. Changes in pH cause varying degrees of attaction and repulsion of 5-prime-AMP and may have been operating on the primitive earth, leading to sequences of adsorption and release of this biomolecule.
Document ID
19850053944
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Banin, A.
(NASA Ames Research Center Extraterrestrial Research Div., Moffett Field, CA; Jerusalem, Hebrew University, Rehovot, Israel)
Lawless, J. G.
(NASA Ames Research Center Moffett Field, CA, United States)
Mazzurco, J.
(NASA Ames Research Center Moffett Field, CA, United States)
Church, F. M.
(NASA Ames Research Center Extraterrestrial Research Div., Moffett Field, CA, United States)
Margulies, L.
(Jerusalem, Hebrew University Rehovot, Israel)
Orenberg, J. B.
(San Francisco State University San Francisco, CA, United States)
Date Acquired
August 12, 2013
Publication Date
January 1, 1985
Publication Information
Publication: Origins of Life
Volume: 15
Issue: 2 19
ISSN: 0302-1688
Subject Category
Space Biology
Accession Number
85A36095
Funding Number(s)
CONTRACT_GRANT: NAGW-094
Distribution Limits
Public
Copyright
Other

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