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Rotating Apparatus for Isoelectric FocusingRemixing of separated fractions prevented. Improved isoelectric focusing apparatus helps to prevent electro-osmosis and convection, both of which cause remixing of separated fractions. Fractionating column segmented and rotated about horizontal axis: Only combined effects of both features fully effective in making good separations. Improved apparatus slowly rotated continuously or rocked (at rotational amplitude of at least 180 degrees) about its horizontal axis so average gravitational vector experienced by fluid is zero and convection is therefore suppressed. Electro-osmosis suppressed and convection further suppressed by separating column into disklike compartments along its length with filters. Experiments have shown dimensions of apparatus not critical. Typical compartment and column volumes are 2 and 40 ml, respectively. Rotation speeds lie between 3 and 30 rpm.
Document ID
19860000308
Acquisition Source
Legacy CDMS
Document Type
Other - NASA Tech Brief
Authors
Bier, M.
(University of Arizona)
Date Acquired
August 12, 2013
Publication Date
May 1, 1986
Publication Information
Publication: NASA Tech Briefs
Volume: 10
Issue: 3
ISSN: 0145-319X
Subject Category
Life Sciences
Report/Patent Number
MFS-26012
Accession Number
86B10308
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.

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