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Higher-order structure of rRNAA comparative search for phylogenetically covarying basepair replacements within potential helices has been the only reliable method to determine the correct secondary structure of the 3 rRNAs, 5S, 16S, and 23S. The analysis of 16S from a wide phylogenetic spectrum, that includes various branches of the eubacteria, archaebacteria, eucaryotes, in addition to the mitochondria and chloroplast, is beginning to reveal the constraints on the secondary structures of these rRNAs. Based on the success of this analysis, and the assumption that higher order structure will also be phylogenetically conserved, a comparative search was initiated for positions that show co-variation not involved in secondary structure helices. From a list of potential higher order interactions within 16S rRNA, two higher-order interactions are presented. The first of these interactions involves positions 570 and 866. Based on the extent of phylogenetic covariation between these positions while maintaining Watson-Crick pairing, this higher-order interaction is considered proven. The other interaction involves a minimum of six positions between the 1400 and 1500 regions of the 16S rRNA. Although these patterns of covariation are not as striking as the 570/866 interaction, the fact that they all exist in an anti-parallel fashion and that experimental methods previously implicated these two regions of the molecule in tRNA function suggests that these interactions be given serious consideration.
Document ID
19860017416
Acquisition Source
Legacy CDMS
Document Type
Conference Paper
Authors
Gutell, R. R.
(Illinois Univ. Urbana, IL, United States)
Woese, C. R.
(Illinois Univ. Urbana, IL, United States)
Date Acquired
August 12, 2013
Publication Date
May 1, 1986
Publication Information
Publication: NASA, Washington Second Symposium on Chemical Evolution and the Origin and Evolution of Life
Subject Category
Space Biology
Accession Number
86N26888
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.
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