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Role of carbonic anhydrase in bone resorption induced by 1,25 dihydroxyvitamin D3 in vitroThe calvaria of 5-to-6-day-old mice treated with 1 x 10 to the -8th M of 1,25(OH)2D3 in vitro for 48 hours are examined in order to study the function of carbonic anhydrase in bone resorption. Calcium concentrations in the culture were measured to assess bone resorption. It is observed that 1,25(OH)2D3 effectively stimulates bone resorption in vitro and the resorption is dose-dependent. The effects of azetazolamide on 1,25(OH)2D3-induced bone resorption are investigated. The data reveal that 1,25(OH)2D3-induced calcium release is associated with an increase in the carbonic anhydrase activity of bone, and bone alkaline phosphatase activity is decreased and acid phosphatase activity is increased in response to 1,25(OH)2D3. A two-fold mechanism for 1,25(OH)2D3-induced bone resorption is proposed; the first mechanism is an indirect activation of osteoclasts and the second involves an interaction between hormone and osteoclast precursors.
Document ID
19860058711
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Hall, G. E.
(Texas Technological Univ. Lubbock, TX, United States)
Kenny, A. D.
(Texas Tech University, Health Science Center Lubbock, United States)
Date Acquired
August 12, 2013
Publication Date
January 1, 1985
Publication Information
Publication: Calcified Tissue International
Volume: 37
ISSN: 0008-0594
Subject Category
Life Sciences (General)
Accession Number
86A43449
Funding Number(s)
CONTRACT_GRANT: NSG-2137
CONTRACT_GRANT: NIH-AM-19475
Distribution Limits
Public
Copyright
Other

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