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An enzyme immunoassay for rat growth hormone - Applications to the study of growth hormone variantsA sensitive and specific competitive enzyme immunoassay for rat growth hormone (GH) is described and its use in the detection of GH variants is demonstrated. In the present assay, soluble GH and GH adsorbed to a solid-phase support compete for monkey anti-GH antibody binding sites. The immobilized antibody-GH complex is detected and quantified using goat antimonkey immunoglobin G covalently conjugated to horseradish peroxidase. It is noted that the assay can be performed in 27 hours and that sensitivities in the range of 0.19 to 25 ng can be obtained in the region of 10 to 90 percent binding.
Document ID
19870066341
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Farrington, Marianne A.
(Pennsylvania State Univ. University Park, PA, United States)
Hymer, W. C.
(Pennsylvania State University University Park, United States)
Date Acquired
August 13, 2013
Publication Date
January 1, 1987
Publication Information
Publication: Life Sciences
Volume: 40
ISSN: 0024-3205
Subject Category
Life Sciences (General)
Accession Number
87A53615
Funding Number(s)
CONTRACT_GRANT: NAS9-17416
CONTRACT_GRANT: NCC2-287
Distribution Limits
Public
Copyright
Other

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