NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
Effect of conductivity and concentration on the sample stream in the transverse axis of a continuous flow electrophoresis chamberThe resolution of continuous flow electrophoresis systems is generally measured by the spread of the sample bands in the direction of the electrophoretic migration. This paper evaluates the cross section of the sample bands in the plane perpendicular to the flow and shows that the spread in the direction perpendicular to the migration increased significantly with the applied electric field. Concentrated samples of monodisperse latex particles and vinyltoluene T-butylstyrene particles in sample buffers of different electrical conductivities were used to map the shape of the sample bands relative to the zero electric field case. As the electric field was applied, the sample band spread from an initial diameter of only one-third the chamber thickness until it approached the chamber walls where electroosmosis significantly reduced the resolution of separation. It can be shown, however, that it is possible to minimize these distortions by careful sample preparation and experiment design.
Document ID
19870066372
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Miller, Teresa Y.
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Williams, George O.
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Snyder, Robert S.
(Calhoun Community College Decatur, AL, United States)
Date Acquired
August 13, 2013
Publication Date
January 1, 1985
Publication Information
Publication: Electrophoresis
Volume: 6
ISSN: 0173-0835
Subject Category
Inorganic And Physical Chemistry
Accession Number
87A53646
Distribution Limits
Public
Copyright
Other

Available Downloads

There are no available downloads for this record.
No Preview Available