Theoretical and experimental separation dynamics in capillary zone electrophoresisThe mathematical model of Bier et al. (1983) is used in a computer aided analysis of the conditions in capillary zone electrophoresis (ZE) under which sample zones migrate noninteractively with the carrier electrolyte. The monitoring of sample zones with a capillary analyzer that features both on-line conductivity and UV detection at the end of the separation trough is discussed. Data from a ZE analysis of a 5-component mixture are presented, and it is noted that all five components can be monitored via their conductivity change if enough sample is present. It is suggested from the results that the concentration ratio of background buffer to sample should be a minimum of 100:1 to effectively apply the plate concept to ZE.
Document ID
19880027096
Acquisition Source
Legacy CDMS
Document Type
Conference Paper
Authors
Thormann, Wolfgang (Arizona Univ. Tucson, AZ, United States)
Michaud, Jon-Pierre (Arizona Univ. Tucson, AZ, United States)
Mosher, Richard A. (Arizona, University Tucson, United States)