NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
Genetic transformation in the methanogen Methanococcus voltae PSMutations causing requirements for histidine, purine, and vitamin B12 were obtained in strain PS of Methanococcus voltae (archaebacteria) upon irradiation with UV or gamma rays. The first two mutations were shown to revert at low frequencies and were used to demonstrate the occurrence of transformation with homologous, wild-type DNA. The transformation rates obtained for these presumably chromosomal markers were in the range of 2 to 100 transformants per microgram of DNA. Mutants resistant to 2-bromoethanesulfonate and to 5-methyl-DL-tryptophan were also isolated.
Document ID
19880033041
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Bertani, G.
(Jet Propulsion Lab., California Inst. of Tech. Pasadena, CA, United States)
Baresi, L.
(California Institute of Technology Jet Propulsion Laboratory, Pasadena, United States)
Date Acquired
August 13, 2013
Publication Date
June 1, 1987
Publication Information
Publication: Journal of Bacteriology
Volume: 169
ISSN: 0021-9193
Subject Category
Life Sciences (General)
Accession Number
88A20268
Distribution Limits
Public
Copyright
Other

Available Downloads

There are no available downloads for this record.
No Preview Available