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Characterization of synthetic carrier ampholytes by repetitive scanning of the electric field during focusingThis paper reports the utilization of a potential gradient array detector for monitoring the dynamics of the electric field during isoelectric focusing. Transient and steady state electric field profiles are presented for synthetic carrier ampholyte mixtures with a wide (about 3-10) pH range. Two available commercial products (Ampholine and Pharmalyte) and a laboratory synthesized mixture (PEHA ampholytes) are compared. The formation of conductivity gaps and their migration toward the cathode in extended experiments (cathodic drift) can be visualized with this system.
Document ID
19880041464
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Thormann, W.
(Arizona Univ. Tucson, AZ, United States)
Egen, N. B.
(Arizona Univ. Tucson, AZ, United States)
Mosher, R. A.
(Arizona Univ. Tucson, AZ, United States)
Bier, M.
(Arizona, University Tucson, United States)
Date Acquired
August 13, 2013
Publication Date
January 1, 1985
Publication Information
Publication: Journal of Biochemical and Biophysical Methods
Volume: 11
ISSN: 0165-022X
Subject Category
Inorganic And Physical Chemistry
Accession Number
88A28691
Funding Number(s)
CONTRACT_GRANT: NSG-7333
CONTRACT_GRANT: NSF CPE-83-11125
Distribution Limits
Public
Copyright
Other

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