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Continuous flow electrophoretic separation of proteins and cells from mammalian tissuesThis paper describes an apparatus for continuous flow electrophoresis (CFE), designed to separate macromolecules and cells at conditions of microgravity. In this CFE, buffer flows upward in a 120-cm long flow chamber, which is 16-cm wide x 3.0-mm thick in the microgravity version (and 6-cm wide x 1.5-mm thick in the unit-gravity laboratory version). Ovalbumin and rat serum albumin were separated in space (flight STS-4) with the same resolution of the two proteins achieved at 25 percent total w/v concentration that was obtained in the laboratory at 0.2 percent w/v concentration. Rat anterior pituitary cells, cultured human embryonic kidney cells, and canine Langerhans cells were separated into subpopulations (flight STS-8) more effectively than in unit gravity, with comparable resolution having been achieved at 100 times the concentration possible on earth.
Document ID
19880041471
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Hymer, W. C.
(Pennsylvania State Univ. University Park, PA, United States)
Barlow, Grant H.
(Pennsylvania State Univ. University Park, PA, United States)
Blaisdell, Steven J.
(Pennsylvania State Univ. University Park, PA, United States)
Cleveland, Carolyn
(Pennsylvania State Univ. University Park, PA, United States)
Farrington, Mary Ann
(Pennsylvania State Univ. University Park, PA, United States)
Feldmeier, Mary
(Pennsylvania State Univ. University Park, PA, United States)
Hatfield, J. Michael
(Pennsylvania State Univ. University Park, PA, United States)
Lanham, J. Wayne
(Pennsylvania State University University Park, United States)
Grindeland, Richard
(NASA Ames Research Center Moffett Field, CA; Pennsylvania State University, University Park, United States)
Snyder, Robert S.
(Marshall Space Flight Center Huntsville, AL; Pennsylvania State University, University Park, United States)
Date Acquired
August 13, 2013
Publication Date
January 1, 1987
Publication Information
Publication: Cell Biophysics
Volume: 10
ISSN: 0163-4992
Subject Category
Inorganic And Physical Chemistry
Accession Number
88A28698
Funding Number(s)
CONTRACT_GRANT: NAS9-15583
CONTRACT_GRANT: NAGW-694
CONTRACT_GRANT: NAS9-15565
CONTRACT_GRANT: PHS-HL-29913
CONTRACT_GRANT: NAS9-16389
CONTRACT_GRANT: PHS-CA-23248
CONTRACT_GRANT: NAS9-17200
CONTRACT_GRANT: PHS-HL-34274
Distribution Limits
Public
Copyright
Other

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