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Transcriptional regulation of decreased protein synthesis during skeletal muscle unloadingThe regulatory role of transcriptional alterations in unloaded skeletal muscles was investigated by determining levels of total muscle RNA and mRNA fractions in soleus, gastrocnemius, and extensor digitorum longus (EDL) of rats subjected to whole-body suspension for up to 7 days. After 7 days, total RNA and mRNA contents were lower in soleus and gastrocnemius, compared with controls, but the concentrations of both RNAs per g muscle were unaltered. Alpha-actin mRNA (assessed by dot hybridization) was significantly reduced in soleus after 1, 3, and 7 days of suspension and in gastrocnemius after 3 and 7 days, but was unchanged in EDL. Protein synthesis directed by RNA extracted from soleus and EDL indicated marked alteration in mRNAs coding for several small proteins. Results suggest that altered transcription and availability of specific mRNAs contribute significantly to the regulation of protein synthesis during skeletal muscle unloading.
Document ID
19890047627
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Howard, G.
(Louisville Univ. KY, United States)
Steffen, J. M.
(Louisville Univ. KY, United States)
Geoghegan, T. E.
(Louisville, University KY, United States)
Date Acquired
August 14, 2013
Publication Date
March 1, 1989
Publication Information
Publication: Journal of Applied Physiology
Volume: 66
ISSN: 0161-7567
Subject Category
Life Sciences (General)
Accession Number
89A34998
Funding Number(s)
CONTRACT_GRANT: NSG-2325
CONTRACT_GRANT: NSF RII-86-10671
Distribution Limits
Public
Copyright
Other

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