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Affinity Electrophoresis Using Ligands Attached To PolymersIn new technique, reduction of electrophoretic mobilities by addition of polyethylene glycol to ligands increases electrophoretic separabilities. In immuno-affinity electrophoresis, modification of ligands extends specificity of electrophoretic separation to particles having surface electric-charge structures otherwise making them electrophoretically inseparable. Modification of antibodies by polyethylene glycol greatly reduces ability to aggregate while enhancing ability to affect electrophoretic mobilities of cells. In hydrophobic-affinity electrophoresis, addition of polyethylene glycol reduces tendency toward aggregation of cells or macromolecules.
Document ID
19900000310
Acquisition Source
Legacy CDMS
Document Type
Other - NASA Tech Brief
Authors
Van Alstine, James M.
(Universities Space Research Association)
Snyder, Robert S.
(Universities Space Research Association)
Harris, J. M.
(Universities Space Research Association)
Brooks, D. E.
(Universities Space Research Association)
Date Acquired
August 14, 2013
Publication Date
June 1, 1990
Publication Information
Publication: NASA Tech Briefs
Volume: 14
Issue: 6
ISSN: 0145-319X
Subject Category
Life Sciences
Report/Patent Number
MFS-26049
Accession Number
90B10310
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.

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