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Investigations on gel forming media use in low gravity bioseparations researchResearch on gelling media and conditions suitable for the preservation of the spatial configuration of cell suspensions and macromolecular solutions after separation in free fluid during low gravity experiments is presented. The examples studied included free electrophoresis of cells in a cylindrical column and two-phase aqueous polymer separation. Microgravity electrophoresis experiments were simulated by separating model cell types (animal or human) in a vertical density gradient containing low-conductivity buffer, 1.7-6.5 percent Ficoll, 6.8-5.0 percent sucrose, and 1 percent SeaPrep low-melting temperature agarose. Upon cooling, a gel formed in the column and cells could be captured at the forming locations. Two-phase extraction experiments were simulated using two-polymer solutions in which phase separation occurs in normal saline at temperatures compatible with cell viability and in which one or both phases form a gel upon cooling. Suitable polymers included commercial agaroses (1-2 percent), maltodextrin (5-7 percent), and gelatin (5-20 percent).
Document ID
19900028011
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Todd, Paul
(National Inst. of Standards and Technology Boulder, CO, United States)
Szlag, David C.
(NIST Boulder, CO, United States)
Plank, Lindsay D.
(National Inst. of Standards and Technology Boulder, CO, United States)
Delcourt, Scott G.
(National Inst. of Standards and Technology Boulder, CO, United States)
Kunze, M. Elaine
(Pennsylvania State University University Park, United States)
Date Acquired
August 14, 2013
Publication Date
January 1, 1989
Publication Information
Publication: - Gravitational Biology, Espoo, Finland, July 18-29, 1988) Advances in Space Research
ISSN: 0273-1177
Subject Category
Materials Processing
Accession Number
90A15066
Funding Number(s)
CONTRACT_GRANT: NAS9-17431
CONTRACT_GRANT: NAS9-15584
Distribution Limits
Public
Copyright
Other

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