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Free flow cell electrophoresis using zwitterionic bufferStudies of a zwitterionic buffer formulated for cell electrophoresis were done using the McDonnell-Douglas Continuous Flow Electrophoresis System. Standard buffers were analyzed for their stability in the electrical field and the results showed that both buffers tested were inherently unstable. Further, titration studies showed that the standards buffers buffered poorly at the pH employed for electrophoresis. The zwitterionic buffer buffered well at its nominal pH and was shown to be stable in the electrical field. Comparative studies of the buffer with standard cell separation buffers using formalin fixed rabbit and goose red blood cells showed that the zwitterionic buffer gave better resolution of the fixed cells. Studies with viable hybridoma cells showed that buffer Q supported cell viability equal to Hank's Balanced Salt Solution and that hybridoma cells in different stages of the growth cycle demonstrated reproducible differences in electrophoretic mobility.
Document ID
19900059587
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Rodkey, R. Scott
(Texas, University Houston, United States)
Date Acquired
August 14, 2013
Publication Date
January 1, 1990
Publication Information
Publication: Applied and Theoretical Electrophoresis
Volume: 1
Issue: 5 19
ISSN: 0954-6642
Subject Category
Materials Processing
Accession Number
90A46642
Funding Number(s)
CONTRACT_GRANT: NAS9-17403
CONTRACT_GRANT: NIH-AI-20590
Distribution Limits
Public
Copyright
Other

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