NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
Protein solubilities determined by a rapid technique and modification of that technique to a micro-methodA simple, rapid method for determination of protein solubilities has been developed which is based upon maximization of the free solution volume to be brought into equilibrium. The tetragonal lysozome solubility diagram has been determined from pH 4.0 to 5.2 (0.1 M sodium acetate), 2-7 percent NaCl, 3-25 C, and portions of the orthorhombic solubility diagram using this technique. Both tetragonal and orthorhombic solubilities were found to increase smoothly with decreasing salt concentration and increasing temperature; no retrograde solubilities were observed. Using column volumes of 75, 300, and 900 microliters, identical tetragonal lysozyme solubility diagrams were obtained. Chymotrypsinogen solubilities have also been determined using this apparatus, being retrograde over the temperature range tested. It is noted that the primary limiting factor in reducing the crystalline volume is the minimum solution sample size needed to accurately quantitate the protein.
Document ID
19910048577
Acquisition Source
Legacy CDMS
Document Type
Conference Paper
Authors
Cacioppo, Elizabeth
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Pusey, Marc Lee
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Munson, Sibyl
(Wisconsin, University Madison, United States)
Date Acquired
August 14, 2013
Publication Date
August 1, 1989
Subject Category
Life Sciences (General)
Accession Number
91A33200
Distribution Limits
Public
Copyright
Other

Available Downloads

There are no available downloads for this record.
No Preview Available