Identification of a far-red reflectance response to ectomycorrhizae in slash pine

Spectral reflectances were measured for the canopies of Pinus elliottii seedlings that were inoculated versus noninoculated with the ectomycorrhizal fungus Pisolithus tinctorius. Reflectances of the less vigorous, noninoculated seedlings increased substantially at visible and near-IR wavelengths compared to the more vigorous, inoculated seedlings. An algorithm was developed to indicate the spectral regions in which reflectance was most sensitive to ectomycorrhizae. The reflectance curve of the more vigorous seedlings was used as a standard of comparison. The spectral regions defined by this algorithm are denoted as regions of maximum reflectance sensitivity. Far-red and near-IR photography filtered to the region of maximum sensitivity (700 nm) were used to enable visualization of a 'blue shift' reflectance response to decreased vigor in the noninoculated seedlings in response to these differences of mycorrhizal development.