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Dexamethasone effects on creatine kinase activity and insulin-like growth factor receptors in cultured muscle cellsThe effect of dexamethasone on the activity of creatine kinase (CK) and the insulin-like growth factor I (IGF-I) binding were investigated using skeletal- and cardiac-muscle-derived cultured cell lines (mouse, C2C12; rat, L6 and H9c2). It was found that, in skeletal muscle cells, dexamethasone treatment during differentiation of skeletal-muscle cells caused dose-dependent increases in CK activity and increases in the degree of myotube formation, whereas cardiac cells (H9c2) exhibited very low CK activity during culture or dexamethasone treatment. Results for IGF-I binding were similar in all three cell lines. The IGF-I binding to dexamethasone-treated cells (50 nM for 24 hr on the day prior to confluence) resulted in an increased number of available binding sites, with no effect on the binding affinities.
Document ID
19920055484
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Whitson, Peggy A.
(NASA Johnson Space Center Houston, TX, United States)
Stuart, Charles A.
(Texas, University Galveston, United States)
Huls, M. H.
(NASA Lyndon B. Johnson Space Center Houston, TX, United States)
Sams, Clarence F.
(NASA Lyndon B. Johnson Space Center Houston, TX, United States)
Cintron, Nitza M.
(NASA Johnson Space Center Houston, TX, United States)
Date Acquired
August 15, 2013
Publication Date
January 1, 1989
Publication Information
Publication: Journal of Cellular Physiology
Volume: 140
ISSN: 0021-9541
Subject Category
Life Sciences (General)
Accession Number
92A38108
Funding Number(s)
CONTRACT_GRANT: NAG9-172
Distribution Limits
Public
Copyright
Other

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