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Photoaffinity labeling of regulatory subunits of protein kinase A in cardiac cell fractions of ratsPhotoaffinity labeling in heart tissue of rats flown on Cosmos 2044 was used to measure the regulatory (R) subunits of adenosine monophosphate-dependent protein kinase. A significant decrease of RII subunits in the particulate cell fraction extract (S2; P less than 0.05 in all cases) was observed when extracts of tissue samples from vivarium controls were compared with those from flight animals. Photoaffinity labeling of the soluble fraction (S1) was observed to be unaffected by spaceflight or any of the simulation conditions. Proteins of the S2 fraction constitute a minor (less than 10 percent) component of the total, whereas the S1 fraction contained most of the cell proteins. Changes in a relatively minor aspect of adenosine monophosphate-mediated reactions are considered to be representative of a metabolic effect.
Document ID
19920068861
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Mednieks, M. I.
(Illinois, University Chicago, United States)
Popova, I.
(Institute of Biomedical Problems Moscow, Russia)
Grindeland, R. E.
(NASA Ames Research Center Moffett Field, CA, United States)
Date Acquired
August 15, 2013
Publication Date
August 1, 1992
Publication Information
Publication: Journal of Applied Physiology, Supplement
Volume: 73
Issue: 2, Au
ISSN: 8750-7587
Subject Category
Life Sciences (General)
Accession Number
92A51485
Distribution Limits
Public
Copyright
Other

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