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Micro-apparatus for rapid determinations of protein solubilitiesWe have developed a column-based micro-technique for rapid determinations of protein solubilities. While retaining a large crystal surface area, the column dead volume has been reduced to equal to or less than 5 micro liters. The technique was tested with tetragonal lysozyme (pH 4.5, 0.1 M acetate, 3.0 percent NaCl, 5-25 C) and column volumes of about 60, 300, and 900 micro liters. Identical solubility data were obtained, indicating that equilibration was obtained even in the smallest columns. In addition, solubility data for Br- and I- salts of lysozyme (pH 4.5, 0.1 M acetate buffer, 0.5 M salt concentrations) were obtained. It appears that the technique can be further miniaturized. The limit in further reducing the crystalline column volume is determined by the minimum solution sample size needed to determine the protein concentration.
Document ID
19930033509
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Pusey, Marc L.
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Munson, Sibyl
(Wisconsin Univ. Madison, United States)
Date Acquired
August 15, 2013
Publication Date
January 1, 1991
Publication Information
Publication: Journal of Crystal Growth
ISSN: 0022-0248
Subject Category
Inorganic And Physical Chemistry
Accession Number
93A17506
Distribution Limits
Public
Copyright
Other

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