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Melatonin and its precursors in Y79 human retinoblastoma cells - Effect of sodium butyrateWe studied the release of melatonin and the production of its precursors, 5-hydroxytryptophan and serotonin, in cultured Y79 human retinoblastoma cells. This biosynthetic capability was found to be dependent on cell differentiation, which was initiated by culturing Y79 cells for 7 days in dishes coated with poly-D-lysine to promote cell adhesion to the surface of the culture dishes. Differentiation was further induced by exposing the cell monolayer to sodium butyrate (3 mM) for three days. This protocol dramatically increased the release of melatonin, and the syntheses of 5-hydroxytryptophan and serotonin in response to forskolin stimulation. Exposure to dopamine or L-DOPA markedly diminished the forskolin-stimulated release of melatonin, as well as the production of 5-hydroxytryptophan and serotonin. These observations indicate that Y79 cells represent a primitive cell line which, following appropriate differentiation can display biochemical characteristics similar to those of the human retina. Moreover, serotonin synthesis and melatonin release appear to be coupled in Y79 ceils.
Document ID
19930048123
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Deng, Mei H.
(NASA Headquarters Washington, DC United States)
Lopez G.-Coviella, Ignacio
(NASA Headquarters Washington, DC United States)
Lynch, Harry J.
(NASA Headquarters Washington, DC United States)
Wurtman, Richard J.
(MIT Cambridge, MA, United States)
Date Acquired
August 16, 2013
Publication Date
January 1, 1991
Publication Information
Publication: Brain Research
ISSN: 0006-8993
Subject Category
Aerospace Medicine
Accession Number
93A32120
Distribution Limits
Public
Copyright
Other

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