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Effect of microgravity on collagenase deprotoeinization and EDTA decalcification of bone fragmentsUndecalcified (n = 140) and decalcified (n = 11) bone fragments were treated with either collagenase (to remove collagen portion; undecalcified n = 64, decalcified n = 11) or EDTA (to remove mineral portion; n= 76) under the reduced gravity environment on US Space Shuttle mission STS-57. The fragments were initially stored in Dulbecco's phosphate buffer solution. After orbit had been established, fragments were exposed to either a neutral buffered collagenase or EDTA solution. Reactions were terminated (neutral buffered formalin for collagenase, 21% CuSO4-5H2O for EDTA) before reentry to earth's atmosphere. Differences in bone samples mass from before flight to after flight were measured. EDTA-treated sample mass was corrected for CuSO4 content. Flight and matched ground (gravitational control) sample showed similar EDTA-induced loss of mineral mass. Collagenase treatments, however, appeared to be more effective in flight samples compared to ground control samples. The flight-exposed, collagenase-treated samples showed significantly more loss than did ground samples. The microgravity environment appeared to promote proteolytic reactions in bone more than the EDTA decalcification reaction.
Document ID
19950041685
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Simske, S. J.
(Univ. of Colorado, Boulder, CO United States)
Luttges, M. W.
(Univ. of Colorado, Boulder, CO United States)
Date Acquired
August 16, 2013
Publication Date
September 1, 1994
Publication Information
Publication: Microgravity Science and Technology
Volume: 7
Issue: 3
ISSN: 0938-0108
Subject Category
Aerospace Medicine
Accession Number
95A73284
Funding Number(s)
CONTRACT_GRANT: NAGW-1197
Distribution Limits
Public
Copyright
Other

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