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Effects of stress ethylene inhibitors on sweet clover (Melilotus Alba L.) seedling growth in microgravityPrevious experiments from our lab have shown that seeds of sweet clover, when germinated and grown within the Fluid Processing Apparatus (FPA) on a slow rotating clinostat produce significantly greater levels of the volatile stress hormone, ethylene, when compared to seeds treated the same but without clinorotation. In both conditions, carbon dioxide levels reached high levels and seedling growth was inhibited. However, clinorotation inhibited growth to a greater extent. To help determine to what extent microgravity influences stress ethylene production and to what extent ethylene inhibits seedling growth, we have extended the above experiments by growing sweet clover in the presence of aminooxyacetic acid (AOA) and silver nitrate (AgNO3), inhibitors of stress ethylene biosynthesis and action, respectively. Seeds of sweet clover were germinated and grown for five days in the FPA under two gravity conditions: under stationary conditions on Earth and in microgravity onboard the space shuttle, Discovery (STS-63), which launched Feb. 3, 1995. Upon recovery, gas samples were aspirated from the growth chambers and carbon dioxide and ethylene concentrations were measured using a gas chromatograph. Then the tissue was weighed, photographed and fixed, and is current undergoing further morphological and microscopic characterization.
Document ID
19960021735
Acquisition Source
Kennedy Space Center
Document Type
Conference Paper
Authors
Gallegos, Gregory L.
(Kansas State Univ. Manhattan, KS United States)
Peterson, Barbara V.
(Dynamac Corp. Cocoa Beach, FL United States)
Brown, Christopher S.
(Dynamac Corp. Cocoa Beach, FL United States)
Guikema, James A.
(Kansas State Univ. Manhattan, KS United States)
Date Acquired
August 17, 2013
Publication Date
January 1, 1995
Publication Information
Publication: Journal of Gravitational Physiology, Volume 2, No. 1
Subject Category
Life Sciences (General)
Accession Number
96N25010
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.
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