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Morphological Analyses of Spring Wheat (CIMMYT cv. PCYT-10) SomaclonesThe objectives of this study were to induce callus from single immature wheat embryos, produce multiple seedlings from the induced callus, and analyse the somaclonal regenerants for potential grain production in a space garden. Immature wheat, Triticum aestivum L. (cv. PCYT-10), embryos were excised 10 to 12 days post-anthesis and cultured on modified Murashige and Skoog's inorganic salts. Embryos cultured on medium containing kinetin (6-furfurylaminopurine) at 0.5mg/l plus 2 or 3mg/l dicamba (1-methoxy-3,6- dichlorobenzoic acid) or 0.2mg/l 2,4-dichlorophenoxyacetic acid produced calli from which 24, 35 and 39% of the explant tissue exhibited regenerants, respectively. The size of flag leaves, plant heights, tillers per plant, spike lengths, awn lengths, and seeds per spike were significantly different in regenerants of two-selfed recurrent generations (SC(sub 1), SC(sub 2)) than in parental controls. However, there were no significant differences in spikelets per spike between the SC(sub 2) and parental controls. Desirable characteristics that were obtained included longer spikes, more seeds per spike, supernumerary spikelets, and larger flag leaves, variants that should be useful in wheat improvement programs.
Document ID
19970028996
Acquisition Source
Ames Research Center
Document Type
Contractor Report (CR)
External Source(s)
Authors
Campbell, W. F.
(Utah State Univ. Logan, UT United States)
Carman, J. G.
(Utah State Univ. Logan, UT United States)
Hashim, Z. N.
(Baghdad Univ. Iraq)
Date Acquired
August 17, 2013
Publication Date
January 1, 1990
Publication Information
Publication: Plant Cell, Tissue and Organ Culture
Publisher: Kluwer Academic Publishers
Volume: 20
Subject Category
Life Sciences (General)
Report/Patent Number
NASA-CR-204935
UAES Paper-3611
NAS 1.26:204395
Accession Number
97N72333
Funding Number(s)
CONTRACT_GRANT: NCC2-139
Distribution Limits
Public
Copyright
Public Use Permitted.
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