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Developmental Changes is Expression of Beta-Adrenergic Receptors in Cultures of C2C12 Skeletal Muscle Cellsbeta-Adrenergic receptor (bAR) agonists have been reported to modulate growth in several mammalian and avian species, and bAR agonists presumably exert their physiological action on skeletal muscle cells through this receptor. Because of the importance of bAR regulation on muscle protein metabolism in muscle cells, the objectives of this study were to determine the developmental expression pattern of the bAR population in C2C12 skeletal muscle cells, and to analyze changes in both the quantity and isoform expression of the major muscle protein, myosin. The number of bAR in mononucleated C2C12 cells was approximately 8,000 bAR per cell, which is comparable with the population reported in several other nonmuscle cell types. However, the bar population increased after myoblast fusion to greater than 50,000 bAR per muscle cell equivalent. The reasons for this apparent over-expression of bAR in C2C12 cells is not known. The quantity of myosin also increased after C2C12 myoblast fusion, but the quantity of myosin was less than that reported in primary muscle cell cultures. Finally, at least five different isoforms of myosin heavy chain could be resolved in C2C12 cells, and three of these exhibited either increased or decreased developmental regulation relative to the others. Thus, C2C12 myoblasts undergo developmental regulation of bAR population and myosin heavy chain isoform expression.
Document ID
20000043993
Acquisition Source
Marshall Space Flight Center
Document Type
Conference Paper
Authors
Young, Ronald B.
(NASA Marshall Space Flight Center Huntsville, AL United States)
Bridge, K. Y.
(NASA Marshall Space Flight Center Huntsville, AL United States)
Vaughn, J. R.
(NASA Marshall Space Flight Center Huntsville, AL United States)
Date Acquired
August 19, 2013
Publication Date
January 1, 2000
Subject Category
Life Sciences (General)
Meeting Information
Meeting: In Vitro Biology Conference
Location: San Diego, CA
Country: United States
Start Date: June 11, 2000
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.

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