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Dynamic Light Scattering of Diabetic VitreopathyDiabetes induces pathology throughout the body via nonenzymatic glycation of proteins. Vitreous, which is replete with type 11 collagen, undergoes significant changes in diabetes. The resultant diabetic vitreopathy plays an important role in diabetic retinopathy. Detecting these molecular changes could provide insight into diabetic eye disease as well as molecular effects elsewhere in the body. Human eyes were obtained at autopsy and studied in the fresh, unfixed state. Sclera, choroid, and retina were dissected off the vitreous for dark-field slit microscopy and dynamic light scattering (DLS). For the former, the entire vitreous was exposed. For the latter, only a window at the equator was dissected in some specimens, and the anterior segment was removed leaving the posterior lens capsule intact in others. DLS was performed to determine particle sizes at multiple sites 0.5 mm apart, spanning the globe at the equator (window dissections) and along the antero-posterior axis. Dark-field slit microscopy in diabetic subjects detected findings typical of age-related vitreous degeneration, but at much younger ages than nondiabetic controls. Noninvasive DLS measurements found a greater heterogeneity and larger particle sizes in vitreous of subjects with diabetes as compared to age-matched controls. DLS can detect and quantify the early molecular effects that cause vitreous collagen fibrils to cross-link and aggregate. This could provide valuable insight into ocular and systemic effects of hyperglycemia, because the molecular changes in diabetic vitreopathy could serve as an index of such effects throughout the body. In addition to the diagnostic implications, this methodology could provide a rapid, reproducible way to monitor the response to therapy with novel agents intended to prevent the complications of diabetes on a molecular level.
Document ID
20010018173
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Sebag, J.
(University of Southern California Los Angeles, CA United States)
Ansari, Rafat R.
(National Center for Microgravity Research on Fluids and Combustion Cleveland, OH United States)
Dunker, Stephan
(University of Southern California Los Angeles, CA United States)
Suh, Kwang I.
(National Center for Microgravity Research on Fluids and Combustion Cleveland, OH United States)
Date Acquired
August 20, 2013
Publication Date
January 1, 1999
Publication Information
Publication: Diabetes Technology and Therapeutics
Publisher: Mary Ann Liebert, Inc.
Volume: 1
Issue: 2
Subject Category
Aerospace Medicine
Distribution Limits
Public
Copyright
Other

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