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Investigating the Effect of Impurities on Macromolecule Crystal Growth in MicrogravityChicken egg-white lysozyme (CEWL) crystals were grown in microgravity and on the ground in the presence of various amounts of a naturally occurring lysozyme dimer impurity. No significant favorable differences in impurity incorporation between microgravity and ground crystal samples were observed. At low impurity concentration the microgravity crystals preferentially incorporated the dimer. The presence of the dimer in the crystallization solutions in microgravity reduced crystal size, increased mosaicity and reduced the signal to noise ratio of the X-ray data. Microgravity samples proved more sensitive to impurity. Accurate indexing of the reflections proved critical to the X-ray analysis. The largest crystals with the best X-ray diffraction properties were grown from pure solution in microgravity.
Document ID
20010037911
Acquisition Source
Marshall Space Flight Center
Document Type
Preprint (Draft being sent to journal)
Authors
Snell, Edward H.
(NASA Marshall Space Flight Center Huntsville, AL United States)
Judge, Russell A.
(NASA Marshall Space Flight Center Huntsville, AL United States)
Crawford, Lisa
(NASA Marshall Space Flight Center Huntsville, AL United States)
Forsythe, Elizabeth L.
(NASA Marshall Space Flight Center Huntsville, AL United States)
Pusey, Marc L.
(NASA Marshall Space Flight Center Huntsville, AL United States)
Sportiello, Michael
(Colorado Univ. Boulder, CO United States)
Todd, Paul
(Colorado Univ. Boulder, CO United States)
Bellamy, Henry
(Stanford Synchrotron Radiation Lab. Menlo Park, CA United States)
Lovelace, Jeff
(Toledo Univ. OH United States)
Cassanto, John M.
(Instrumentation Technology Associates, Inc. Exton, PA United States)
Rose, M. Franklin
Date Acquired
August 20, 2013
Publication Date
January 1, 2001
Subject Category
Space Processing
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.

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