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Comparison of Light Emitting Diodes (LED) and Fluorescent Light on Suppression of Pineal Melatonin in the RatTo validate a novel LED array for use in animal habitat lighting by comparing its effectiveness to cool-white fluorescent (CWF) lighting in suppressing pineal gland melatonin. Male Sprague-Dawley rats, 175-200 g, were maintained under control conditions for 2 weeks (food and water ad lib, 12L: 12D CWF, 18 uW/square cm). Dark adapted animals (animals before lights on) were exposed to 5 min of LED or CWF light of similar spectral power distribution. Two groups of rats (LED vs. CWF) were compared at 5 light intensities (100, 40, 1, 1.0, and 0. 1 lux). A control group was placed into the exposure apparatus but not exposed to light. After exposure, pineal glands were rapidly removed and assayed for melatonin by RIA. Results. The dark-exposed control groups matched with the 5 intensity groups (100, 40, 10, 1.0, and 0.1 lux) showed mean + SEM pineal melatonin values of 1167 +/- 136, 1569 +/- 126, 353 +/- 34, 650 +/- 124, and 464 +/- 85, pg/ml respectively. The corresponding CWF exposure data were 393 1 41, 365 +34, 257 +/- 13, 218 +/- 42, and 239 +/- 71 pg/ml, respectively. Corresponding LED exposure data were 439 +/- 25, 462 +/- 50, 231 +/- 6, 164 +/- 12, and 158 +/- 12 pg/ml, respectively. Rats exposed to both experimental light conditions at all illuminances studied showed significant melatonin suppression (p less than 0.01, ANOVA). In no case was the melatonin suppression induced by LED illuminance significantly different from the melatonin suppression elicited by the same intensity of CWF light. The results show that a novel LED light source can suppress pineal melatonin equal to that of a conventional CWF light source.
Document ID
20020042356
Acquisition Source
Ames Research Center
Document Type
Conference Paper
Authors
Winget, Charles M.
(NASA Ames Research Center Moffett Field, CA United States)
Heeke, D. S.
Holley, D. C.
Mele, G.
Brainard, G. C.
Hanifin, J. P.
Rollag, M. D.
Savage, Paul D.
Date Acquired
August 20, 2013
Publication Date
January 1, 1997
Subject Category
Electronics And Electrical Engineering
Meeting Information
Meeting: International Congress on Chronobiology
Location: Paris
Country: France
Start Date: September 7, 1997
End Date: September 11, 1997
Funding Number(s)
PROJECT: RTOP 106-50-06-40
CONTRACT_GRANT: NCC2-779
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.

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