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Two-Photon Fluorescence Correlation SpectroscopyWe will describe a two-photon microscope currently under development at the NASA Glenn Research Center. It is composed of a Coherent Mira 900 tunable, pulsed Titanium:Sapphire laser system, an Olympus Fluoview 300 confocal scanning head, and a Leica DM IRE inverted microscope. It will be used in conjunction with a technique known as fluorescence correlation spectroscopy (FCS) to study intracellular protein dynamics. We will briefly explain the advantages of the two-photon system over a conventional confocal microscope, and provide some preliminary experimental results.
Document ID
20030005598
Acquisition Source
Glenn Research Center
Document Type
Conference Paper
Authors
Zimmerli, Gregory A. (NASA Glenn Research Center Cleveland, OH United States)
Fischer, David G. (NASA Glenn Research Center Cleveland, OH United States)
Date Acquired
August 21, 2013
Publication Date
November 1, 2002
Publication Information
Publication: Sixth Microgravity Fluid Physics and Transport Phenomena Conference: Exposition Topical Areas 1-6
Volume: 2
Subject Category
Instrumentation And Photography
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.
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