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Tindallia californiensis sp. nov., a new anaerobic, haloalkaliphilic, spore-forming acetogen isolated from Mono Lake in CaliforniaA novel extremely haloalkaliphilic, strictly anaerobic, acetogenic bacterium strain APO was isolated from sediments of the athalassic, meromictic, alkaline Mono Lake in California. The Gram-positive, spore-forming, slightly curved rods with sizes 0.55- 0.7x1.7-3.0 microns were motile by a single laterally attached flagellum. Strain APO was mesophilic (range 10-48 C, optimum of 37 C); halophilic (NaCl range 1-20% (w/v) with optimum of 3-5% (w/v), and alkaliphilic (pH range 8.0-10.5, optimum 9.5). The novel isolate required sodium ions in the medium. Strain APO was an organotroph with a fermentative type of metabolism and used the substrates peptone, bacto-tryptone, casamino acid, yeast extract, L-serine, L-lysine, L-histidine, L-arginine, and pyruvate. The new isolate performed the Stickland reaction with the following amino acid pairs: proline + alanine, glycine + alanine, and tryptophan + valine. The main end product of growth was acetate. High activity of CO dehydrogenase and hydrogenase indicated the presence of a homoacetogenic, non-cycling acetyl-coA pathway. Strain APO was resistant to kanamycin but sensitive to chloramphenicol, tetracycline, and gentamycin. The G+C content of the genomic DNA was 44.4 mol% (by HPLC method). The sequence of the 16s rRNA gene of strain APO possessed 98.2% similarity with the sequence from Tindullia magadiensis Z-7934, but the DNA-DNA hybridization value between these organisms was only 55%. On the basis of these physiological and molecular properties, strain APO is proposed to be a novel species of the genus Tindallia with the name Tindallia californiensis sp. nov., (type strain APO = ATCC BAA-393 - DSM 14871).
Document ID
20030113142
Acquisition Source
Marshall Space Flight Center
Document Type
Reprint (Version printed in journal)
Authors
Pikuta, E. V.
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Hoover, R. B.
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Bej, A. K.
(Alabama Univ. Birmingham, AL, United States)
Marsic, D.
(Alabama Univ. Birmingham, AL, United States)
Detkova, E. N.
(Academy of Sciences (USSR) Moscow, USSR)
Whitman, W. B.
(Georgia Univ. Athens, GA, United States)
Krader, P.
(American Type Culture Collection Manassas, VA, United States)
Date Acquired
August 21, 2013
Publication Date
May 1, 2003
Publication Information
Publication: Extremophiles
Volume: 7
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other

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