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Diffraction and Imaging Study of Imperfections of Protein Crystals with Coherent X-raysHigh angular-resolution x-ray diffraction and phase contrast x-ray imaging were combined to study defects and perfection of protein crystals. Imperfections including line defects, inclusions and other microdefects were observed in the diffraction images of a uniformly grown lysozyme crystal. The observed line defects carry distinct dislocation features running approximately along the <110> growth front and have been found to originate mostly in a central growth area and occasionally in outer growth regions. Slow dehydration led to the broadening of a fairly symmetric 4 4 0 rocking curve by a factor of approximately 2.6, which was primarily attributed to the dehydration-induced microscopic effects that are clearly shown in diffraction images. X-ray imaging and diffraction characterization of the quality of apoferritin crystals will also be discussed in the presentation.
Document ID
20040085898
Acquisition Source
Marshall Space Flight Center
Document Type
Conference Paper
Authors
Hu, Z. W.
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Thomas, B. R.
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Chernov, A. A.
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Chu, Y. S.
(Argonne National Lab. IL, United States)
Lai, B.
(Argonne National Lab. IL, United States)
Date Acquired
August 21, 2013
Publication Date
January 1, 2004
Subject Category
Solid-State Physics
Meeting Information
Meeting: American Crystallographic Association Annual Meeting
Location: Chicago, IL
Country: United States
Start Date: July 17, 2004
End Date: July 22, 2004
Funding Number(s)
CONTRACT_GRANT: NAS8-02096
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.

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