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Intricate Crystal Structure of Dihydrolipoamide Dehydrogenase (E3) with its Binding Protein: Multiple Copies, Dynamic and Static DisordersHuman E3 and binding protein E3BP are two components of the pyruvate dehydrogenase complex. Crystallization of E3 with 221-amino acid fragment of E3BP (E3BPdd) led to crystals that diffracted to a resolution of 2.6 Angstroms. Structure determination involved molecular replacement using a dimer of E3 homolog as a search model and de novo building of the E3BPdd peptide. Solution was achieved by inclusion of one E3 dimer at a time, followed by refinement until five E3 dimers were located. This complete content of E3 provided electron density maps suitable for tracing nine peptide chains of E3BPdd, eight of them being identified with partial occupancies. Final content of the asymmetric unit consists of five E3 dimers, each binding one E3BPdd molecule. In four of these molecular complexes, E3BPdd is in static disorder resulting in E3BPdd binding to either one or the other monomer of the E3 dimer. However, E3BPdd of the fifth E3 dimer forms specific contacts that lock it at one monomer. In addition to this static disorder, E3BPdd reveals high mobility in the limited space of the crystal lattice. Support from NIH and NASA.
Document ID
20040085934
Acquisition Source
Marshall Space Flight Center
Document Type
Preprint (Draft being sent to journal)
Authors
Makal, A.
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Hong, Y. S.
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Potter, R.
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Vettaikkorumakankauv, A. K.
(State Univ. of New York Buffalo, NY, United States)
Korotchkina, L. G.
(State Univ. of New York Buffalo, NY, United States)
Patel, M. S.
(State Univ. of New York Buffalo, NY, United States)
Ciszak, E.
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Date Acquired
August 21, 2013
Publication Date
January 1, 2004
Subject Category
Inorganic, Organic And Physical Chemistry
Meeting Information
Meeting: American Crystallographic Association
Location: Chicago, IL
Country: United States
Start Date: July 17, 2004
End Date: July 22, 2004
Sponsors: American Crystallographic Association
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.

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